A concise guide to choosing suitable gene expression systems for recombinant protein production

Anja Schütz; Frank Bernhard; Nick Berrow; Johannes F. Buyel; Frederico Ferreira-da-Silva; Jurgen Haustraete; Joop van den Heuvel; Jan Erik Hoffmann; Ario de Marco; Yoav Peleg; Sabine Suppmann; Tamar Unger; Martine Vanhoucke; Susanne Witt; Kim Remans

doi:10.1016/j.xpro.2023.102572

A concise guide to choosing suitable gene expression systems for recombinant protein production

Anja Schütz, Frank Bernhard, Nick Berrow, Johannes F. Buyel, Frederico Ferreira-da-Silva, Jurgen Haustraete, Joop van den Heuvel, Jan Erik Hoffmann, Ario de Marco, Yoav Peleg, Sabine Suppmann, Tamar Unger, Martine Vanhoucke, Susanne Witt, Kim Remans^*

^*Corresponding author for this work

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Research output: Contribution to journal › Review article › peer-review

9 Citations (Scopus)

Abstract

This overview guides both novices and experienced researchers facing challenging targets to select the most appropriate gene expression system for producing a particular protein. By answering four key questions, readers can determine the most suitable gene expression system following a decision scheme. This guide addresses the most commonly used and accessible systems and provides brief descriptions of the main gene expression systems’ key characteristics to assist decision making. Additionally, information has been included for selected less frequently used “exotic” gene expression systems.

Original language	English
Article number	102572
Journal	STAR Protocols
Volume	4
Issue number	4
Early online date	31 Oct 2023
DOIs	https://doi.org/10.1016/j.xpro.2023.102572
Publication status	Published - 15 Dec 2023

All Science Journal Classification (ASJC) codes

General Neuroscience
General Biochemistry,Genetics and Molecular Biology
General Immunology and Microbiology

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10.1016/j.xpro.2023.102572Licence: CC BY-NC-ND

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Schütz, A., Bernhard, F., Berrow, N., Buyel, J. F., Ferreira-da-Silva, F., Haustraete, J., van den Heuvel, J., Hoffmann, J. E., de Marco, A., Peleg, Y., Suppmann, S., Unger, T., Vanhoucke, M., Witt, S., & Remans, K. (2023). A concise guide to choosing suitable gene expression systems for recombinant protein production. STAR Protocols, 4(4), Article 102572. https://doi.org/10.1016/j.xpro.2023.102572

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Schütz, A, Bernhard, F, Berrow, N, Buyel, JF, Ferreira-da-Silva, F, Haustraete, J, van den Heuvel, J, Hoffmann, JE, de Marco, A, Peleg, Y, Suppmann, S, Unger, T, Vanhoucke, M, Witt, S & Remans, K 2023, 'A concise guide to choosing suitable gene expression systems for recombinant protein production', STAR Protocols, vol. 4, no. 4, 102572. https://doi.org/10.1016/j.xpro.2023.102572

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T1 - A concise guide to choosing suitable gene expression systems for recombinant protein production

AU - Schütz, Anja

AU - Bernhard, Frank

AU - Berrow, Nick

AU - Buyel, Johannes F.

AU - Ferreira-da-Silva, Frederico

AU - Haustraete, Jurgen

AU - van den Heuvel, Joop

AU - Hoffmann, Jan Erik

AU - de Marco, Ario

AU - Peleg, Yoav

AU - Suppmann, Sabine

AU - Unger, Tamar

AU - Vanhoucke, Martine

AU - Witt, Susanne

AU - Remans, Kim

PY - 2023/12/15

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N2 - This overview guides both novices and experienced researchers facing challenging targets to select the most appropriate gene expression system for producing a particular protein. By answering four key questions, readers can determine the most suitable gene expression system following a decision scheme. This guide addresses the most commonly used and accessible systems and provides brief descriptions of the main gene expression systems’ key characteristics to assist decision making. Additionally, information has been included for selected less frequently used “exotic” gene expression systems.

AB - This overview guides both novices and experienced researchers facing challenging targets to select the most appropriate gene expression system for producing a particular protein. By answering four key questions, readers can determine the most suitable gene expression system following a decision scheme. This guide addresses the most commonly used and accessible systems and provides brief descriptions of the main gene expression systems’ key characteristics to assist decision making. Additionally, information has been included for selected less frequently used “exotic” gene expression systems.

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JO - STAR Protocols

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A concise guide to choosing suitable gene expression systems for recombinant protein production

Abstract

All Science Journal Classification (ASJC) codes

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