A framework for neural organoids, assembloids and transplantation studies

Sergiu P. Pașca; Paola Arlotta; Helen S. Bateup; J. Gray Camp; Silvia Cappello; Fred H. Gage; Jürgen A. Knoblich; Arnold R. Kriegstein; Madeline A. Lancaster; Guo Li Ming; Gaia Novarino; Hideyuki Okano; Malin Parmar; In Hyun Park; Orly Reiner; Hongjun Song; Lorenz Studer; Jun Takahashi; Sally Temple; Giuseppe Testa; Barbara Treutlein; Flora M. Vaccarino; Pierre Vanderhaeghen; Tracy Young-Pearse

doi:10.1038/s41586-024-08487-6

A framework for neural organoids, assembloids and transplantation studies

Sergiu P. Pașca^*, Paola Arlotta, Helen S. Bateup, J. Gray Camp, Silvia Cappello, Fred H. Gage, Jürgen A. Knoblich, Arnold R. Kriegstein, Madeline A. Lancaster, Guo Li Ming, Gaia Novarino, Hideyuki Okano, Malin Parmar, In Hyun Park, Orly Reiner, Hongjun Song, Lorenz Studer, Jun Takahashi, Sally Temple, Giuseppe TestaBarbara Treutlein, Flora M. Vaccarino, Pierre Vanderhaeghen, Tracy Young-Pearse

^*Corresponding author for this work

Department of Molecular Genetics

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

As the field of neural organoids and assembloids expands, there is an emergent need for guidance and advice on designing, conducting and reporting experiments to increase the reproducibility and utility of these models. In this Perspective, we present a framework for the experimental process that encompasses ensuring the quality and integrity of human pluripotent stem cells, characterizing and manipulating neural cells in vitro, transplantation techniques and considerations for modelling human development, evolution and disease. As with all scientific endeavours, we advocate for rigorous experimental designs tailored to explicit scientific questions as well as transparent methodologies and data sharing to provide useful knowledge for current research practices and for developing regulatory standards.

Original language	English
Pages (from-to)	315-320
Number of pages	6
Journal	Nature
Volume	639
Issue number	8054
Early online date	9 Dec 2024
DOIs	https://doi.org/10.1038/s41586-024-08487-6
Publication status	Published - 13 Mar 2025

Funding

he authors thank members of their laboratories who provided feedback on earlier versions of this manuscript, including A. Jourdon, V. Mariano, T. L. Li, N. Caporale, E. Villa and M. Sutcliffe.

All Science Journal Classification (ASJC) codes

General

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Pașca, S. P., Arlotta, P., Bateup, H. S., Camp, J. G., Cappello, S., Gage, F. H., Knoblich, J. A., Kriegstein, A. R., Lancaster, M. A., Ming, G. L., Novarino, G., Okano, H., Parmar, M., Park, I. H., Reiner, O., Song, H., Studer, L., Takahashi, J., Temple, S., ... Young-Pearse, T. (2025). A framework for neural organoids, assembloids and transplantation studies. Nature, 639(8054), 315-320. https://doi.org/10.1038/s41586-024-08487-6

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title = "A framework for neural organoids, assembloids and transplantation studies",

abstract = "As the field of neural organoids and assembloids expands, there is an emergent need for guidance and advice on designing, conducting and reporting experiments to increase the reproducibility and utility of these models. In this Perspective, we present a framework for the experimental process that encompasses ensuring the quality and integrity of human pluripotent stem cells, characterizing and manipulating neural cells in vitro, transplantation techniques and considerations for modelling human development, evolution and disease. As with all scientific endeavours, we advocate for rigorous experimental designs tailored to explicit scientific questions as well as transparent methodologies and data sharing to provide useful knowledge for current research practices and for developing regulatory standards.",

author = "Pașca, {Sergiu P.} and Paola Arlotta and Bateup, {Helen S.} and Camp, {J. Gray} and Silvia Cappello and Gage, {Fred H.} and Knoblich, {J{\"u}rgen A.} and Kriegstein, {Arnold R.} and Lancaster, {Madeline A.} and Ming, {Guo Li} and Gaia Novarino and Hideyuki Okano and Malin Parmar and Park, {In Hyun} and Orly Reiner and Hongjun Song and Lorenz Studer and Jun Takahashi and Sally Temple and Giuseppe Testa and Barbara Treutlein and Vaccarino, {Flora M.} and Pierre Vanderhaeghen and Tracy Young-Pearse",

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doi = "10.1038/s41586-024-08487-6",

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Pașca, SP, Arlotta, P, Bateup, HS, Camp, JG, Cappello, S, Gage, FH, Knoblich, JA, Kriegstein, AR, Lancaster, MA, Ming, GL, Novarino, G, Okano, H, Parmar, M, Park, IH, Reiner, O, Song, H, Studer, L, Takahashi, J, Temple, S, Testa, G, Treutlein, B, Vaccarino, FM, Vanderhaeghen, P & Young-Pearse, T 2025, 'A framework for neural organoids, assembloids and transplantation studies', Nature, vol. 639, no. 8054, pp. 315-320. https://doi.org/10.1038/s41586-024-08487-6

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AU - Pașca, Sergiu P.

AU - Arlotta, Paola

AU - Bateup, Helen S.

AU - Camp, J. Gray

AU - Cappello, Silvia

AU - Gage, Fred H.

AU - Knoblich, Jürgen A.

AU - Kriegstein, Arnold R.

AU - Lancaster, Madeline A.

AU - Ming, Guo Li

AU - Novarino, Gaia

AU - Okano, Hideyuki

AU - Parmar, Malin

AU - Park, In Hyun

AU - Reiner, Orly

AU - Song, Hongjun

AU - Studer, Lorenz

AU - Takahashi, Jun

AU - Temple, Sally

AU - Testa, Giuseppe

AU - Treutlein, Barbara

AU - Vaccarino, Flora M.

AU - Vanderhaeghen, Pierre

AU - Young-Pearse, Tracy

PY - 2025/3/13

Y1 - 2025/3/13

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ER -

A framework for neural organoids, assembloids and transplantation studies

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