Abstract
The firing of neurons throughout the brain is determined by the precise relations between excitatory and inhibitory inputs, and disruption of their balance underlies many psychiatric diseases. Whether or not these inputs covary over time or between repeated stimuli remains unclear due to the lack of experimental methods for measuring both inputs simultaneously. We developed a new analytical framework for instantaneous and simultaneous measurements of both the excitatory and inhibitory neuronal inputs during a single trial under current clamp recording. This can be achieved by injecting a current composed of two high frequency sinusoidal components followed by analytical extraction of the conductances. We demonstrate the ability of this method to measure both inputs in a single trial under realistic recording constraints and from morphologically realistic CA1 pyramidal model cells. Future experimental implementation of our new method will facilitate the understanding of fundamental questions about the health and disease of the nervous system.
| Original language | English |
|---|---|
| Article number | e1009725 |
| Number of pages | 24 |
| Journal | PLoS Computational Biology |
| Volume | 17 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - 28 Dec 2021 |
Funding
Funding: This work was supported by grants from the DFG-SFB 1089 (to HB and IL), 01EW1606 - DeCipher EraNet Neuron (to HB and IL), Israel Science Foundation (ISF 1539/17 and ISF Bikura 2799/20) (to IL), Minerva Foundation (to IL) and Human Frontier Science Program (HFSP) (to IL). I would like to thank Hagay Famini for checking and testing some computational aspects that were used in this study and to Michael Sokoletsly for outstanding comments on the manuscript. We thank Dr. Michael Okun for his comments on the early version of the manuscript and for providing the simulated data used in Fig 6 and to Dr. Alexander Binshtok and Ben Title for providing the data used in Fig 7 and their important comments on this study..