Application of a "top-down" approach for the analysis of selective Fe+2 -catalyzed oxidative cleavages of Na.K-ATPase from pig kidney by mass spectrometry. Implication for the energy transduction mechanism

R. Goldshleger; G. Pachornik; T. Mehlman; M. Benjamin; D. Merhav; S. J.D. Karlish; A. Shainskaya

Application of a "top-down" approach for the analysis of selective Fe⁺² -catalyzed oxidative cleavages of Na.K-ATPase from pig kidney by mass spectrometry. Implication for the energy transduction mechanism

R. Goldshleger^*, G. Pachornik, T. Mehlman, M. Benjamin, D. Merhav, S. J.D. Karlish, A. Shainskaya

^*Corresponding author for this work

Department of Biomolecular Sciences

Research output: Contribution to conference › Paper › peer-review

2 Citations (Scopus)

Abstract

The structure-function relations of renal Na/K-ATPase or gastric H,K-ATpase were studied using transition metal (FE or Cu)-catalyzed oxidative cleavage. This technique provides information on the spatial organization in the different conformational states of the important membrane protein. Cleavaged dependent on ATP-Fe²⁺ reveal positions of Mg²⁺ in the active site and their changes in different states of the catalytic cycle. MS measurements after electroelution or extraction of hydrophobic proteins from gels, make this a much more powerful technique than hitherto, and should have many other important applications.

Original language	English
Pages	103-104
Number of pages	2
Publication status	Published - 2002
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: 2 Jun 2002 → 6 Jun 2002

Conference

Conference	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	2/6/02 → 6/6/02

All Science Journal Classification (ASJC) codes

Spectroscopy

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Goldshleger, R., Pachornik, G., Mehlman, T., Benjamin, M., Merhav, D., Karlish, S. J. D., & Shainskaya, A. (2002). Application of a "top-down" approach for the analysis of selective Fe⁺² -catalyzed oxidative cleavages of Na.K-ATPase from pig kidney by mass spectrometry. Implication for the energy transduction mechanism. 103-104. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

Goldshleger, R. ; Pachornik, G. ; Mehlman, T. et al. / Application of a "top-down" approach for the analysis of selective Fe⁺² -catalyzed oxidative cleavages of Na.K-ATPase from pig kidney by mass spectrometry. Implication for the energy transduction mechanism. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.2 p.

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abstract = "The structure-function relations of renal Na/K-ATPase or gastric H,K-ATpase were studied using transition metal (FE or Cu)-catalyzed oxidative cleavage. This technique provides information on the spatial organization in the different conformational states of the important membrane protein. Cleavaged dependent on ATP-Fe2+ reveal positions of Mg2+ in the active site and their changes in different states of the catalytic cycle. MS measurements after electroelution or extraction of hydrophobic proteins from gels, make this a much more powerful technique than hitherto, and should have many other important applications.",

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Goldshleger, R, Pachornik, G, Mehlman, T, Benjamin, M, Merhav, D, Karlish, SJD & Shainskaya, A 2002, 'Application of a "top-down" approach for the analysis of selective Fe⁺² -catalyzed oxidative cleavages of Na.K-ATPase from pig kidney by mass spectrometry. Implication for the energy transduction mechanism', Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 2/6/02 - 6/6/02 pp. 103-104.

Application of a "top-down" approach for the analysis of selective Fe⁺² -catalyzed oxidative cleavages of Na.K-ATPase from pig kidney by mass spectrometry. Implication for the energy transduction mechanism. / Goldshleger, R.; Pachornik, G.; Mehlman, T. et al.
2002. 103-104 Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

Research output: Contribution to conference › Paper › peer-review

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AU - Goldshleger, R.

AU - Pachornik, G.

AU - Mehlman, T.

AU - Benjamin, M.

AU - Merhav, D.

AU - Karlish, S. J.D.

AU - Shainskaya, A.

PY - 2002

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N2 - The structure-function relations of renal Na/K-ATPase or gastric H,K-ATpase were studied using transition metal (FE or Cu)-catalyzed oxidative cleavage. This technique provides information on the spatial organization in the different conformational states of the important membrane protein. Cleavaged dependent on ATP-Fe2+ reveal positions of Mg2+ in the active site and their changes in different states of the catalytic cycle. MS measurements after electroelution or extraction of hydrophobic proteins from gels, make this a much more powerful technique than hitherto, and should have many other important applications.

AB - The structure-function relations of renal Na/K-ATPase or gastric H,K-ATpase were studied using transition metal (FE or Cu)-catalyzed oxidative cleavage. This technique provides information on the spatial organization in the different conformational states of the important membrane protein. Cleavaged dependent on ATP-Fe2+ reveal positions of Mg2+ in the active site and their changes in different states of the catalytic cycle. MS measurements after electroelution or extraction of hydrophobic proteins from gels, make this a much more powerful technique than hitherto, and should have many other important applications.

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T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

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Goldshleger R, Pachornik G, Mehlman T, Benjamin M, Merhav D, Karlish SJD et al.. Application of a "top-down" approach for the analysis of selective Fe⁺² -catalyzed oxidative cleavages of Na.K-ATPase from pig kidney by mass spectrometry. Implication for the energy transduction mechanism. 2002. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.