Abstract
How cells diversify to form an embryo represents a profound interdisciplinary challenge. Decades of innovative research using model organisms have uncovered principles of lineage specification, morphogenesis, epigenetic mechanisms, and gene regulation that underlie this fundamental process. As biology enters the genomic era, marked by rapid convergence of technological and computational advances, construction of quantitative and heuristic models of development becomes increasingly feasible. In gastrulation, a founding population of equipotent stem cells rapidly diversifies in a highly canonical manner to form the basic body plan. This review discusses considerations required to establish a time-resolved model that reflects the cellular and molecular aspects involved in this process. Building on insights from recent studies and the transformative potential of evolving technologies and experimental frameworks, we discuss how to devise such a model by integrating multiple molecular modalities at the single-cell level within the spatial context as a benchmark for studying cell specification.
| Original language | English |
|---|---|
| Pages (from-to) | 135-158 |
| Number of pages | 24 |
| Journal | Annual Review of Cell and Developmental Biology |
| Volume | 41 |
| Issue number | 1 |
| Early online date | 4 Jun 2025 |
| DOIs | |
| Publication status | Published - 1 Oct 2025 |
Funding
We apologize to the authors we could not cite due to space limitations. We thank the Stelzer lab for valuable discussions and Shlomit Edri, Raz Ben-Yair, and Ilana Taieb-Hagerty for comments on the manuscript. Y.S is the incumbent of the Louis and Ida Rich Career Development Chair. Research in the Stelzer lab is supported by a European Research Council Consolidator Grant (EmbryoCellEnsemble 101123880), the Israel Science Foundation (2824/24), and research grants from the Estate of Betty Weneser and the Estate of Hermine Miller.
All Science Journal Classification (ASJC) codes
- Developmental Biology
- Cell Biology