Contrast enhanced photoacoustic detection of fibrillar collagen in the near infrared region-I

Inna Solomonov; Irene Locatelli; Silvia Tortorella; Manu Unni; Shay Lee Aharoni; Elisa Alchera; Erica Locatelli; Mirko Maturi; Chiara Venegoni; Roberta Lucianò; Andrea Salonia; Angelo Corti; Flavio Curnis; Valeria Grasso; Gayathri Malamal; Jithin Jose; Mauro Comes Franchini; Irit Sagi; Massimo Alfano

doi:10.1039/d4na00204k

Contrast enhanced photoacoustic detection of fibrillar collagen in the near infrared region-I

Inna Solomonov, Irene Locatelli, Silvia Tortorella, Manu Unni, Shay Lee Aharoni, Elisa Alchera, Erica Locatelli, Mirko Maturi, Chiara Venegoni, Roberta Lucianò, Andrea Salonia, Angelo Corti, Flavio Curnis, Valeria Grasso, Gayathri Malamal, Jithin Jose, Mauro Comes Franchini^*, Irit Sagi^*, Massimo Alfano^*

^*Corresponding author for this work

Department of Immunology and Regenerative Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Fibrillar collagen accumulation emerges as a promising biomarker in several diseases, such as desmoplastic tumors and unstable atherosclerotic plaque. Gold nanorods (GNRs) hold great potential as contrast agents in high-resolution, biomedically safe, and non-invasive photoacoustic imaging (PAI). This study presents the design and characterization of a specialized imaging tool which exploits GNR assisted targeted photoacoustic imaging that is tailored for the identification of fibrillar collagen. In addition to the photoacoustic characterization of collagen in the NIR 1 and 2 regions, we demonstrate the detailed steps of conjugating a decoy to GNRs. This study serves as a proof of concept, that demonstrates that conjugated collagenase-1 (MMP-1) generates a distinct and collagen-specific photoacoustic signal, facilitating real-time visualization in the wavelength range of 700-970 nm (NIR I). As most of the reported studies utilized the endogenous contrast of collagen in the NIR II wavelength that has major limitations to perform in vivo deep tissue imaging, the approach that we are proposing is unique and it highlights the promise of MMP-1 decoy-functionalized GNRs as novel contrast agents for photoacoustic imaging of collagen in the NIR 1 region. To our knowledge this is the first time functionalized GNRs are optimized for the detection of fibrillar collagen and utilized in the field of non-invasive photoacoustic imaging that can facilitate a better prognosis of desmoplastic tumors and broken atherosclerotic plaques.

Original language	English
Pages (from-to)	3655-3667
Number of pages	13
Journal	Nanoscale Advances
Volume	6
Issue number	14
DOIs	https://doi.org/10.1039/d4na00204k
Publication status	Published Online - 7 Jun 2024

Bibliographical note

We thank Dr Y. Fridmann Sirkis and the Protein Analysis Unit for suggestions. The EM studies were conducted at the Moskowitz Center for Nano and Bio-Nano Imaging at the Weizmann Institute of Science. This study has received funding from the European Union's Horizon 2020 research and innovation program under grant agreement no. 801126 (https://cordis.europa.eu/project/id/801126) and from the Horizon Europe framework programme under grant agreement no. 101113193 (https://cordis.europa.eu/project/id/101113193). The funding source had no role in the design of this study, data interpretation and writing of the report.

Publisher Copyright:
© 2024 RSC.

All Science Journal Classification (ASJC) codes

Bioengineering
Atomic and Molecular Physics, and Optics
General Chemistry
General Materials Science
General Engineering

Access to Document

10.1039/d4na00204kLicence: CC BY-NC

Cite this

Solomonov, I., Locatelli, I., Tortorella, S., Unni, M., Aharoni, S. L., Alchera, E., Locatelli, E., Maturi, M., Venegoni, C., Lucianò, R., Salonia, A., Corti, A., Curnis, F., Grasso, V., Malamal, G., Jose, J., Comes Franchini, M., Sagi, I., & Alfano, M. (2024). Contrast enhanced photoacoustic detection of fibrillar collagen in the near infrared region-I. Nanoscale Advances, 6(14), 3655-3667. Advance online publication. https://doi.org/10.1039/d4na00204k

@article{cefcf754131043b99afadf2458f18561,

title = "Contrast enhanced photoacoustic detection of fibrillar collagen in the near infrared region-I",

abstract = "Fibrillar collagen accumulation emerges as a promising biomarker in several diseases, such as desmoplastic tumors and unstable atherosclerotic plaque. Gold nanorods (GNRs) hold great potential as contrast agents in high-resolution, biomedically safe, and non-invasive photoacoustic imaging (PAI). This study presents the design and characterization of a specialized imaging tool which exploits GNR assisted targeted photoacoustic imaging that is tailored for the identification of fibrillar collagen. In addition to the photoacoustic characterization of collagen in the NIR 1 and 2 regions, we demonstrate the detailed steps of conjugating a decoy to GNRs. This study serves as a proof of concept, that demonstrates that conjugated collagenase-1 (MMP-1) generates a distinct and collagen-specific photoacoustic signal, facilitating real-time visualization in the wavelength range of 700-970 nm (NIR I). As most of the reported studies utilized the endogenous contrast of collagen in the NIR II wavelength that has major limitations to perform in vivo deep tissue imaging, the approach that we are proposing is unique and it highlights the promise of MMP-1 decoy-functionalized GNRs as novel contrast agents for photoacoustic imaging of collagen in the NIR 1 region. To our knowledge this is the first time functionalized GNRs are optimized for the detection of fibrillar collagen and utilized in the field of non-invasive photoacoustic imaging that can facilitate a better prognosis of desmoplastic tumors and broken atherosclerotic plaques.",

author = "Inna Solomonov and Irene Locatelli and Silvia Tortorella and Manu Unni and Aharoni, {Shay Lee} and Elisa Alchera and Erica Locatelli and Mirko Maturi and Chiara Venegoni and Roberta Lucian{\`o} and Andrea Salonia and Angelo Corti and Flavio Curnis and Valeria Grasso and Gayathri Malamal and Jithin Jose and {Comes Franchini}, Mauro and Irit Sagi and Massimo Alfano",

note = "We thank Dr Y. Fridmann Sirkis and the Protein Analysis Unit for suggestions. The EM studies were conducted at the Moskowitz Center for Nano and Bio-Nano Imaging at the Weizmann Institute of Science. This study has received funding from the European Union's Horizon 2020 research and innovation program under grant agreement no. 801126 (https://cordis.europa.eu/project/id/801126) and from the Horizon Europe framework programme under grant agreement no. 101113193 (https://cordis.europa.eu/project/id/101113193). The funding source had no role in the design of this study, data interpretation and writing of the report. Publisher Copyright: {\textcopyright} 2024 RSC.",

year = "2024",

month = jun,

day = "7",

doi = "10.1039/d4na00204k",

language = "English",

volume = "6",

pages = "3655--3667",

journal = "Nanoscale Advances",

issn = "2516-0230",

publisher = "Royal Society of Chemistry",

number = "14",

}

Solomonov, I, Locatelli, I, Tortorella, S, Unni, M, Aharoni, SL, Alchera, E, Locatelli, E, Maturi, M, Venegoni, C, Lucianò, R, Salonia, A, Corti, A, Curnis, F, Grasso, V, Malamal, G, Jose, J, Comes Franchini, M, Sagi, I & Alfano, M 2024, 'Contrast enhanced photoacoustic detection of fibrillar collagen in the near infrared region-I', Nanoscale Advances, vol. 6, no. 14, pp. 3655-3667. https://doi.org/10.1039/d4na00204k

TY - JOUR

T1 - Contrast enhanced photoacoustic detection of fibrillar collagen in the near infrared region-I

AU - Solomonov, Inna

AU - Locatelli, Irene

AU - Tortorella, Silvia

AU - Unni, Manu

AU - Aharoni, Shay Lee

AU - Alchera, Elisa

AU - Locatelli, Erica

AU - Maturi, Mirko

AU - Venegoni, Chiara

AU - Lucianò, Roberta

AU - Salonia, Andrea

AU - Corti, Angelo

AU - Curnis, Flavio

AU - Grasso, Valeria

AU - Malamal, Gayathri

AU - Jose, Jithin

AU - Comes Franchini, Mauro

AU - Sagi, Irit

AU - Alfano, Massimo

N1 - We thank Dr Y. Fridmann Sirkis and the Protein Analysis Unit for suggestions. The EM studies were conducted at the Moskowitz Center for Nano and Bio-Nano Imaging at the Weizmann Institute of Science. This study has received funding from the European Union's Horizon 2020 research and innovation program under grant agreement no. 801126 (https://cordis.europa.eu/project/id/801126) and from the Horizon Europe framework programme under grant agreement no. 101113193 (https://cordis.europa.eu/project/id/101113193). The funding source had no role in the design of this study, data interpretation and writing of the report. Publisher Copyright: © 2024 RSC.

PY - 2024/6/7

Y1 - 2024/6/7

N2 - Fibrillar collagen accumulation emerges as a promising biomarker in several diseases, such as desmoplastic tumors and unstable atherosclerotic plaque. Gold nanorods (GNRs) hold great potential as contrast agents in high-resolution, biomedically safe, and non-invasive photoacoustic imaging (PAI). This study presents the design and characterization of a specialized imaging tool which exploits GNR assisted targeted photoacoustic imaging that is tailored for the identification of fibrillar collagen. In addition to the photoacoustic characterization of collagen in the NIR 1 and 2 regions, we demonstrate the detailed steps of conjugating a decoy to GNRs. This study serves as a proof of concept, that demonstrates that conjugated collagenase-1 (MMP-1) generates a distinct and collagen-specific photoacoustic signal, facilitating real-time visualization in the wavelength range of 700-970 nm (NIR I). As most of the reported studies utilized the endogenous contrast of collagen in the NIR II wavelength that has major limitations to perform in vivo deep tissue imaging, the approach that we are proposing is unique and it highlights the promise of MMP-1 decoy-functionalized GNRs as novel contrast agents for photoacoustic imaging of collagen in the NIR 1 region. To our knowledge this is the first time functionalized GNRs are optimized for the detection of fibrillar collagen and utilized in the field of non-invasive photoacoustic imaging that can facilitate a better prognosis of desmoplastic tumors and broken atherosclerotic plaques.

AB - Fibrillar collagen accumulation emerges as a promising biomarker in several diseases, such as desmoplastic tumors and unstable atherosclerotic plaque. Gold nanorods (GNRs) hold great potential as contrast agents in high-resolution, biomedically safe, and non-invasive photoacoustic imaging (PAI). This study presents the design and characterization of a specialized imaging tool which exploits GNR assisted targeted photoacoustic imaging that is tailored for the identification of fibrillar collagen. In addition to the photoacoustic characterization of collagen in the NIR 1 and 2 regions, we demonstrate the detailed steps of conjugating a decoy to GNRs. This study serves as a proof of concept, that demonstrates that conjugated collagenase-1 (MMP-1) generates a distinct and collagen-specific photoacoustic signal, facilitating real-time visualization in the wavelength range of 700-970 nm (NIR I). As most of the reported studies utilized the endogenous contrast of collagen in the NIR II wavelength that has major limitations to perform in vivo deep tissue imaging, the approach that we are proposing is unique and it highlights the promise of MMP-1 decoy-functionalized GNRs as novel contrast agents for photoacoustic imaging of collagen in the NIR 1 region. To our knowledge this is the first time functionalized GNRs are optimized for the detection of fibrillar collagen and utilized in the field of non-invasive photoacoustic imaging that can facilitate a better prognosis of desmoplastic tumors and broken atherosclerotic plaques.

UR - http://www.scopus.com/inward/record.url?scp=85195851186&partnerID=8YFLogxK

U2 - 10.1039/d4na00204k

DO - 10.1039/d4na00204k

M3 - Article

AN - SCOPUS:85195851186

SN - 2516-0230

VL - 6

SP - 3655

EP - 3667

JO - Nanoscale Advances

JF - Nanoscale Advances

IS - 14

ER -

Contrast enhanced photoacoustic detection of fibrillar collagen in the near infrared region-I

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this