Design and synthesis of piperine-based photoaffinity probes for revealing potential targets of piperine in neurological disease

Li Shen, Yue Yang, Lijun Lu, Jili Huang, Wen He, Chunfang Zhao, Feng Guo, Chunbo Zhang*, Haijun Zhong*, Fan Liao*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Piperine (PIP) has attracted extensive attention due to its diverse biological activities. In this study, we developed two photoaffinity probes PIP-1 and PIP-2, which are biologically safe and retain PIP's bioactivity, to investigate its protein targets in vivo. Using in situ labeling and cell imaging, we were able to effectively detect and visualize the drug targets of PIP with our probes. Additionally, a series of protein targets of PIP were fished using PIP-2 through proteome profiling, with further validation suggesting that TGFβ1 might be a potential target involved in PIP's effects on neurological diseases. These findings demonstrate that PIP-2 is a valuable tool for identifying the targets of PIP.

Original languageEnglish
Pages (from-to)669-672
Number of pages4
JournalChemical Communications
Volume61
Issue number4
DOIs
Publication statusPublished Online - 29 Nov 2024

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Catalysis
  • Ceramics and Composites
  • General Chemistry
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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