Dissecting Human Blood Immune Cells Response to Intracellular Infection Using Single-Cell RNA Sequencing

Shelly Hen-Avivi; Roi Avraham

doi:10.1007/978-1-0716-1971-1_12

Dissecting Human Blood Immune Cells Response to Intracellular Infection Using Single-Cell RNA Sequencing

Shelly Hen-Avivi, Roi Avraham^*

^*Corresponding author for this work

Department of Immunology and Regenerative Biology

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Complex interactions between diverse host immune cells can determine the outcome of pathogen infections. Advances in single-cell RNA sequencing (scRNA-seq) allow detection of the transcriptional patterns of different immune cells at steady state and after infection. To reveal the complex interactions of the human immune system in response to diverse intracellular pathogens, we developed a protocol for scRNA-seq of ex vivo infected human peripheral blood mononuclear cells (PBMCs). We demonstrate here infection with Salmonella enterica serovar Typhimurium, but this protocol can be used for any other pathogen of interest, and expand our knowledge of human host–pathogen biology.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press
Pages	133-147
Number of pages	15
ISBN (Print)	978-1-0716-1970-4
DOIs	https://doi.org/10.1007/978-1-0716-1971-1_12
Publication status	Published Online - 27 May 2022

Publication series

Series	Methods in Molecular Biology
ISSN	1064-3745

Bibliographical note

Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

All Science Journal Classification (ASJC) codes

Genetics
Molecular Biology

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10.1007/978-1-0716-1971-1_12

Cite this

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Dissecting Human Blood Immune Cells Response to Intracellular Infection Using Single-Cell RNA Sequencing

Abstract

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