Abstract
Motivation: Unlike many other families of enzymes, which catalyze the same overall reaction, aminoacyl-tRNA synthetases (aaRSs) are extremely heterogeneous in terms of primary sequence and subunit organization. For the most part aaRSs are negatively charged at physiological conditions, as are tRNA substrates. What are the driving forces that ensure an attraction between like-charged macromolecules? As may be inferred from multiple sequence alignments (MSA), concentration of the invariant charged residues in structural domains doesn't correlate with contribution of the domains to formation of the electrostatic field at long distances. Results: In aaRSs family the subset of evolutionary non-conserved charged residues generates long-range electrostatic potential (EP) similar to the native one. We evaluate contribution of individual structural domains to the EP generated by native (NS), conservative (CS) and non-conservative subsets (NCS) of charged residues. For monomeric IIeRS and heterodimeric PheRS we further analyzed the interplay between the domain functionality and their role in the field formation at long distances.
| Original language | English |
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| Pages (from-to) | 310-314 |
| Number of pages | 5 |
| Journal | Proceedings Of The Fifth International Conference On Bioinformatics Of Genome Regulation And Structure, Vol 1 |
| Publication status | Published - 2006 |
| Event | 5th International Conference on Bioinformatics of Genome Regulation and Structure - Novosibirsk, RUSSIA Duration: 16 Jul 2006 → 22 Jul 2006 |