Abstract
Human Embryonic Stem (hES) cells are a potential large-scale source of neural cells that could be used for transplantation to regenerate neural functions, including myelination, affected by disease or injury. Starting from huES cells, we derived highly enriched populations of glial precursors that can be expanded and passaged repeatedly, and after growth factor (GF) removal, progressively differentiate into 04 positive highly branched human oligodendrocytes, and later into myelinating cells. The gene expression profile shows enrichment of growing cells in oligodendroglial progenitors (OGP), which express Olig 1/2, Nkx2.2, but not Sox10. Cells after GF withdrawal, as they become 04 positive oligodendrocyte precursors (OPC), acquire first the expression of Sox10 mRNA, and increase the myelin gene PLP mRNA. The myelin gene MBP mRNA appears later. In co-culture with neurons from mouse dorsal root ganglia, the hES-derived OPC are seen to migrate, differentiate and interact with axons.
| Original language | English |
|---|---|
| Pages (from-to) | 3-11 |
| Number of pages | 9 |
| Journal | Proceedings Of The Viii European Meeting On Glial Cells In Health And Disease |
| Publication status | Published - 2007 |
| Event | 8th European Meeting on Glial Cells in Health and Disease - London, ENGLAND Duration: 4 Sept 2007 → 8 Sept 2007 |