Immune related proteins and tumor infiltrating CD8+lymphocytes in hypopharyngeal cancer in relation to human papillomavirus (HPV) and clinical outcome

David Landin; Andreas Ahrlund-Richter; Leila Mirzaie; Michael Mints; Anders Nasman; Aeneas Kolev; Linda Marklund; Tina Dalianis; Eva Munck-Wikland; Torbjorn Ramqvist

doi:10.1002/hed.26364

Immune related proteins and tumor infiltrating CD8+lymphocytes in hypopharyngeal cancer in relation to human papillomavirus (HPV) and clinical outcome

David Landin, Andreas Ahrlund-Richter, Leila Mirzaie, Michael Mints, Anders Nasman, Aeneas Kolev, Linda Marklund, Tina Dalianis, Eva Munck-Wikland, Torbjorn Ramqvist^*

^*Corresponding author for this work

Department of Molecular Cell Biology

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Background - Hypopharyngeal cancer (HPSCC) shows a poor clinical outcome, while HPSCC, caused by human papillomavirus (HPV), presents a better outcome. Here, HPCC, immune proteins, and tumor infiltrating CD8+ lymphocytes (CD8+ TILs) were evaluated in relation to HPV and outcome. Methods - Fresh frozen tissue from four HPV-positive HPSCC, 39 HPV-negative HPSCC, and normal samples were analyzed for protein expression by the Proseek immuno-oncology immunoassay. CD8+ TIL numbers evaluated by immunohistochemistry on 144 formalin-fixed biopsies were analyzed in relation to clinical outcome. Results - Proteins differing between HPV-positive and negative HPSCC included CD8A, PD-L1, Fas ligand, and chemokines. High CD8+ TIL numbers were correlated to improve clinical outcome in HPV-negative HPSCC. Conclusions - High expression of immune proteins in HPV-positive HPSCC may explain the better clinical outcome. CD8+ TILs are of relevance for outcome of HPV-negative HPSCC, while tumors with high immune activity but poor patient survival suggest a role for immune therapy.

Original language	English
Pages (from-to)	3206-3217
Number of pages	12
Journal	Head and Neck
Volume	42
Issue number	11
Early online date	1 Jul 2020
DOIs	https://doi.org/10.1002/hed.26364
Publication status	Published - Nov 2020

Funding

The authors would like to acknowledge support of the Clinical biomarker facility at SciLifeLab Sweden for providing assistance in protein analysis. We thank Susanne Becker, Inger Bodin, and Anna Malmerfelt for excellent technical assistance and Ola Hössjer for advice regarding data analysis. This work was supported by the Cancer Society in Stockholm (151253, 181053), The Swedish Cancer Foundation (180440), the Stockholm County ALF (20180085, 2018037), Laryngfonden and the Cancer and Allergy Foundation (253). Anders Näsman was supported by Region Stockholm (clinical postdoctoral appointment). The funding sources had no involvement in any part of the study.

All Science Journal Classification (ASJC) codes

Otorhinolaryngology

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title = "Immune related proteins and tumor infiltrating CD8+lymphocytes in hypopharyngeal cancer in relation to human papillomavirus (HPV) and clinical outcome",

abstract = "Background - Hypopharyngeal cancer (HPSCC) shows a poor clinical outcome, while HPSCC, caused by human papillomavirus (HPV), presents a better outcome. Here, HPCC, immune proteins, and tumor infiltrating CD8+ lymphocytes (CD8+ TILs) were evaluated in relation to HPV and outcome. Methods - Fresh frozen tissue from four HPV-positive HPSCC, 39 HPV-negative HPSCC, and normal samples were analyzed for protein expression by the Proseek immuno-oncology immunoassay. CD8+ TIL numbers evaluated by immunohistochemistry on 144 formalin-fixed biopsies were analyzed in relation to clinical outcome. Results - Proteins differing between HPV-positive and negative HPSCC included CD8A, PD-L1, Fas ligand, and chemokines. High CD8+ TIL numbers were correlated to improve clinical outcome in HPV-negative HPSCC. Conclusions - High expression of immune proteins in HPV-positive HPSCC may explain the better clinical outcome. CD8+ TILs are of relevance for outcome of HPV-negative HPSCC, while tumors with high immune activity but poor patient survival suggest a role for immune therapy.",

author = "David Landin and Andreas Ahrlund-Richter and Leila Mirzaie and Michael Mints and Anders Nasman and Aeneas Kolev and Linda Marklund and Tina Dalianis and Eva Munck-Wikland and Torbjorn Ramqvist",

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pages = "3206--3217",

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Landin, D, Ahrlund-Richter, A, Mirzaie, L, Mints, M, Nasman, A, Kolev, A, Marklund, L, Dalianis, T, Munck-Wikland, E & Ramqvist, T 2020, 'Immune related proteins and tumor infiltrating CD8+lymphocytes in hypopharyngeal cancer in relation to human papillomavirus (HPV) and clinical outcome', Head and Neck, vol. 42, no. 11, pp. 3206-3217. https://doi.org/10.1002/hed.26364

TY - JOUR

T1 - Immune related proteins and tumor infiltrating CD8+lymphocytes in hypopharyngeal cancer in relation to human papillomavirus (HPV) and clinical outcome

AU - Landin, David

AU - Ahrlund-Richter, Andreas

AU - Mirzaie, Leila

AU - Mints, Michael

AU - Nasman, Anders

AU - Kolev, Aeneas

AU - Marklund, Linda

AU - Dalianis, Tina

AU - Munck-Wikland, Eva

AU - Ramqvist, Torbjorn

PY - 2020/11

Y1 - 2020/11

N2 - Background - Hypopharyngeal cancer (HPSCC) shows a poor clinical outcome, while HPSCC, caused by human papillomavirus (HPV), presents a better outcome. Here, HPCC, immune proteins, and tumor infiltrating CD8+ lymphocytes (CD8+ TILs) were evaluated in relation to HPV and outcome. Methods - Fresh frozen tissue from four HPV-positive HPSCC, 39 HPV-negative HPSCC, and normal samples were analyzed for protein expression by the Proseek immuno-oncology immunoassay. CD8+ TIL numbers evaluated by immunohistochemistry on 144 formalin-fixed biopsies were analyzed in relation to clinical outcome. Results - Proteins differing between HPV-positive and negative HPSCC included CD8A, PD-L1, Fas ligand, and chemokines. High CD8+ TIL numbers were correlated to improve clinical outcome in HPV-negative HPSCC. Conclusions - High expression of immune proteins in HPV-positive HPSCC may explain the better clinical outcome. CD8+ TILs are of relevance for outcome of HPV-negative HPSCC, while tumors with high immune activity but poor patient survival suggest a role for immune therapy.

AB - Background - Hypopharyngeal cancer (HPSCC) shows a poor clinical outcome, while HPSCC, caused by human papillomavirus (HPV), presents a better outcome. Here, HPCC, immune proteins, and tumor infiltrating CD8+ lymphocytes (CD8+ TILs) were evaluated in relation to HPV and outcome. Methods - Fresh frozen tissue from four HPV-positive HPSCC, 39 HPV-negative HPSCC, and normal samples were analyzed for protein expression by the Proseek immuno-oncology immunoassay. CD8+ TIL numbers evaluated by immunohistochemistry on 144 formalin-fixed biopsies were analyzed in relation to clinical outcome. Results - Proteins differing between HPV-positive and negative HPSCC included CD8A, PD-L1, Fas ligand, and chemokines. High CD8+ TIL numbers were correlated to improve clinical outcome in HPV-negative HPSCC. Conclusions - High expression of immune proteins in HPV-positive HPSCC may explain the better clinical outcome. CD8+ TILs are of relevance for outcome of HPV-negative HPSCC, while tumors with high immune activity but poor patient survival suggest a role for immune therapy.

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Immune related proteins and tumor infiltrating CD8+lymphocytes in hypopharyngeal cancer in relation to human papillomavirus (HPV) and clinical outcome

Abstract

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