Abstract
Single cell intracellular recordings in-vivo at deep brain structures are seldomly accompanied by nearby optogenetics or drug application. The use of such tools is limited as both light and drugs cannot penetrate deep inside brain tissue. Hence, the optical fiber or drug delivery pipette needs to be placed within the brain close to the recording pipette. So far, however, this has required highly accurate hardware to achieve. These complications have now been solved by new approaches enabling intracellular recordings both for optogenetics and pharmacological application by the use of a single manipulator. In this manuscript we review these technologies - their pros, cons and implications.
| Original language | English |
|---|---|
| Article number | 108324 |
| Number of pages | 6 |
| Journal | Journal of Neuroscience Methods |
| Volume | 325 |
| Early online date | 6 Jul 2019 |
| DOIs | |
| Publication status | Published - 1 Sept 2019 |
Funding
Ilan Lampl is the incumbent of the Norman and Helen Asher Professorial Chair at the Weizmann Institute of Science. Yonatan Katz is incumbent of the Marianne Manoville Beck Research Fellow Chair in Brain Research. This research was supported by DFG (SFB 1089), EraNet (DeCipher Neuron 01EW1606), Human Frontier Science Program Grant, Israel Science Foundation (ISF 1539/17), Minerva, and the Marianne Manoville Beck Laboratory for Research in Neurobiology in Honor of her Parents Elisabeth and Miksa Manoville, all awarded to I.L..