Introduction of correlative light and airSEM™ microscopy imaging for tissue research under ambient conditions

Inna Solomonov; Dalit Talmi-Frank; Yonat Milstein; Sefi Addadi; Anna Aloshin; Irit Sagi

doi:10.1038/srep05987

Introduction of correlative light and airSEM™ microscopy imaging for tissue research under ambient conditions

Inna Solomonov, Dalit Talmi-Frank, Yonat Milstein, Sefi Addadi, Anna Aloshin, Irit Sagi

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

A complete fingerprint of a tissue sample requires a detailed description of its cellular and extracellular components while minimizing artifacts. We introduce the application of a novel scanning electron microscope (airSEM™) in conjunction with light microscopy for functional analysis of tissue preparations at nanometric resolution (<10 nm) and under ambient conditions. Our metal-staining protocols enable easy and detailed visualization of tissues and their extracellular scaffolds. A multimodality imaging setup, featuring airSEM™ and a light microscope on the same platform, provides a convenient and easy-to-use system for obtaining structural and functional correlative data. The airSEM™ imaging station complements other existing imaging solutions and shows great potential for studies of complex biological systems.

Original language	English
Article number	5987
Journal	Scientific Reports
Volume	4
DOIs	https://doi.org/10.1038/srep05987
Publication status	Published - 7 Aug 2014

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abstract = "A complete fingerprint of a tissue sample requires a detailed description of its cellular and extracellular components while minimizing artifacts. We introduce the application of a novel scanning electron microscope (airSEM{\texttrademark}) in conjunction with light microscopy for functional analysis of tissue preparations at nanometric resolution (<10 nm) and under ambient conditions. Our metal-staining protocols enable easy and detailed visualization of tissues and their extracellular scaffolds. A multimodality imaging setup, featuring airSEM{\texttrademark} and a light microscope on the same platform, provides a convenient and easy-to-use system for obtaining structural and functional correlative data. The airSEM{\texttrademark} imaging station complements other existing imaging solutions and shows great potential for studies of complex biological systems.",

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T1 - Introduction of correlative light and airSEM™ microscopy imaging for tissue research under ambient conditions

AU - Solomonov, Inna

AU - Talmi-Frank, Dalit

AU - Milstein, Yonat

AU - Addadi, Sefi

AU - Aloshin, Anna

AU - Sagi, Irit

PY - 2014/8/7

Y1 - 2014/8/7

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AB - A complete fingerprint of a tissue sample requires a detailed description of its cellular and extracellular components while minimizing artifacts. We introduce the application of a novel scanning electron microscope (airSEM™) in conjunction with light microscopy for functional analysis of tissue preparations at nanometric resolution (<10 nm) and under ambient conditions. Our metal-staining protocols enable easy and detailed visualization of tissues and their extracellular scaffolds. A multimodality imaging setup, featuring airSEM™ and a light microscope on the same platform, provides a convenient and easy-to-use system for obtaining structural and functional correlative data. The airSEM™ imaging station complements other existing imaging solutions and shows great potential for studies of complex biological systems.

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JO - Scientific Reports

JF - Scientific Reports

M1 - 5987

ER -

Introduction of correlative light and airSEM™ microscopy imaging for tissue research under ambient conditions

Abstract

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