Metrizamide density gradients of sea urchin chromatin: fractions rich and poor in nascent DNA

Avraham Levy; Karl M. Jakob; Boaz Moav

doi:10.1093/nar/2.12.2299

Metrizamide density gradients of sea urchin chromatin: fractions rich and poor in nascent DNA

Avraham Levy, Karl M. Jakob, Boaz Moav

Department of Plant and Environmental Sciences

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

A crude, lightly sheared chromatin preparation obtained from a mixture of[methyl-3H] thymidine pulse and [2-14C] thymidine long-labeled sea urchin embryos (swimming blastulae), was centrifuged in metrizamide to form an isopycnic gradient. The buoyant density of the 3H pulse labeled chromatin was slightly higher than that of the 14C labeled bulk chromatin. The 3H/14C ratios in the higher and lower density regions of the overlapping radioactivity peaks, indicated the presence of fractions rich and poor in nascent DNA in these two density regions. After 15 min chase, the difference disappeared, indicating that the chromatin fractions with nascent DNA have a half-life shorter than 15 min.

Original language	English
Pages (from-to)	2299-2304
Number of pages	6
Journal	Nucleic Acids Research
Volume	2
Issue number	12
DOIs	https://doi.org/10.1093/nar/2.12.2299
Publication status	Published - Dec 1975

Funding

The active participation of Israel Tal in this work is gratefully acknowledged. K.M. Jakob holds a Michael and Audrey Sacher Staff Fellowship.

All Science Journal Classification (ASJC) codes

Genetics

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abstract = "A crude, lightly sheared chromatin preparation obtained from a mixture of[methyl-3H] thymidine pulse and [2-14C] thymidine long-labeled sea urchin embryos (swimming blastulae), was centrifuged in metrizamide to form an isopycnic gradient. The buoyant density of the 3H pulse labeled chromatin was slightly higher than that of the 14C labeled bulk chromatin. The 3H/14C ratios in the higher and lower density regions of the overlapping radioactivity peaks, indicated the presence of fractions rich and poor in nascent DNA in these two density regions. After 15 min chase, the difference disappeared, indicating that the chromatin fractions with nascent DNA have a half-life shorter than 15 min.",

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N2 - A crude, lightly sheared chromatin preparation obtained from a mixture of[methyl-3H] thymidine pulse and [2-14C] thymidine long-labeled sea urchin embryos (swimming blastulae), was centrifuged in metrizamide to form an isopycnic gradient. The buoyant density of the 3H pulse labeled chromatin was slightly higher than that of the 14C labeled bulk chromatin. The 3H/14C ratios in the higher and lower density regions of the overlapping radioactivity peaks, indicated the presence of fractions rich and poor in nascent DNA in these two density regions. After 15 min chase, the difference disappeared, indicating that the chromatin fractions with nascent DNA have a half-life shorter than 15 min.

AB - A crude, lightly sheared chromatin preparation obtained from a mixture of[methyl-3H] thymidine pulse and [2-14C] thymidine long-labeled sea urchin embryos (swimming blastulae), was centrifuged in metrizamide to form an isopycnic gradient. The buoyant density of the 3H pulse labeled chromatin was slightly higher than that of the 14C labeled bulk chromatin. The 3H/14C ratios in the higher and lower density regions of the overlapping radioactivity peaks, indicated the presence of fractions rich and poor in nascent DNA in these two density regions. After 15 min chase, the difference disappeared, indicating that the chromatin fractions with nascent DNA have a half-life shorter than 15 min.

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Metrizamide density gradients of sea urchin chromatin: fractions rich and poor in nascent DNA

Abstract

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