Multi-well fibrillation assay as a tool for analyzing crosslinking and stabilization of collagen

Venkat Raghavan Krishnaswamy*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

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Abstract

Collagen is the most widely used substratum in cell culture and biomaterials applications. In this chapter, we describe a simple procedure to isolate collagen, which can be employed to a wide range of tissue sources, and subsequently use it to study the collagen crosslinking and stabilization abilities of various compounds. The protocol is designed for a multi-well format assay and thus can be used for simultaneous assessment of multiple number of compounds and can be easily adapted to a high-throughput screening setup.

Original languageEnglish
Title of host publicationCollagen
Subtitle of host publicationMethods and Protocols
EditorsIrit Sagi, Nikolaos A Afratis
PublisherHumana Press
Pages17-22
Number of pages6
ISBN (Electronic)978-1-4939-9095-5
ISBN (Print)978-1-4939-9094-8
DOIs
Publication statusPublished Online - 7 Mar 2019

Publication series

SeriesMethods in Molecular Biology
Volume1944
ISSN1064-3745

Bibliographical note

NA

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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