Abstract
Recent developments in high-throughput sequencing and genome editing enable breeders to use a new tool kit for precision breeding. DNA double strand breaks can be targeted to desired genomic loci by custom-designed nucleases such as Zinc-finger nucleases, TALEN and CRISPR-Cas9. The repair products of induced breaks vary depending on the repair mechanisms and the repair template. These may be targeted small insertions or deletions, gene conversions or crossovers as well as gene replacement. In this chapter we review the concepts, achievements, opportunities and challenges of these new technologies.
Original language | English |
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Title of host publication | Plant Biotechnology : Experience and Future Prospects |
Pages | 39-49 |
Number of pages | 11 |
ISBN (Electronic) | 9783030683450 |
DOIs | |
Publication status | Published Online - 31 Aug 2021 |