Reinvestigation of carbohydrate specificity of ebca-1 monoclonal antibody used for the detection of candida mannan

Vadim B. Krylov, Arsenii S. Solovev, Ilya A. Puchkin, Dmitry V. Yashunsky, Anna V. Antonets, Olga Y. Kutsevalova, Nikolay E. Nifantiev*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Monoclonal antibody EBCA-1 is used in the sandwich immune assay for the detection of circulating Candida mannan in blood sera samples for the diagnosis of invasive candidiasis. To reinvestigate carbohydrate specificity of EBCA-1, a panel of biotinylated oligosaccharides structurally related to distinct fragments of Candida mannan were loaded onto a streptavidin-coated plate to form a glycoarray. Its use demonstrated that EBCA-1 recognizes the trisaccharide β-Man-(1→2)-α-Man-(1→2)-α-Man and not homo-α-(1→2)-linked pentamannoside, as was reported previously.

Original languageEnglish
Article number504
JournalJournal of Fungi
Volume7
Issue number7
DOIs
Publication statusPublished - Jul 2021
Externally publishedYes

Funding

Funding: Scientific Schools Development Program of N.D. Zelinsky Institute of Organic Chemistry, RAS is gratefully acknowledged.

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science
  • Microbiology (medical)

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