Abstract
Monoclonal antibody EBCA-1 is used in the sandwich immune assay for the detection of circulating Candida mannan in blood sera samples for the diagnosis of invasive candidiasis. To reinvestigate carbohydrate specificity of EBCA-1, a panel of biotinylated oligosaccharides structurally related to distinct fragments of Candida mannan were loaded onto a streptavidin-coated plate to form a glycoarray. Its use demonstrated that EBCA-1 recognizes the trisaccharide β-Man-(1→2)-α-Man-(1→2)-α-Man and not homo-α-(1→2)-linked pentamannoside, as was reported previously.
Original language | English |
---|---|
Article number | 504 |
Journal | Journal of Fungi |
Volume | 7 |
Issue number | 7 |
DOIs | |
Publication status | Published - Jul 2021 |
Externally published | Yes |
Funding
Funding: Scientific Schools Development Program of N.D. Zelinsky Institute of Organic Chemistry, RAS is gratefully acknowledged.
All Science Journal Classification (ASJC) codes
- Ecology, Evolution, Behavior and Systematics
- Plant Science
- Microbiology (medical)