Single-Molecule Fluorescent In Situ Hybridization (smFISH) for RNA Detection in Bacteria

Camilla Ciolli Mattioli; Roi Avraham

doi:10.1007/978-1-0716-3766-1_1

Single-Molecule Fluorescent In Situ Hybridization (smFISH) for RNA Detection in Bacteria

Camilla Ciolli Mattioli, Roi Avraham

Department of Immunology and Regenerative Biology

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

In this chapter, we describe in detail how to perform a successful smFISH experiment and how to quantify mRNA transcripts in bacterial cells. The flexibility of the method allows for straightforward adaptation to different bacterial species and experimental conditions. Thanks to the feasibility of the approach, the method can easily be adapted by other laboratories. Finally, we believe that this method has a great potential to generate insights into the complicated life of bacteria.

Original language	English
Title of host publication	Fluorescence In Situ Hybridization (FISH)
Subtitle of host publication	Methods and Protocols
Editors	Gal Haimovich
Publisher	Humana Press
Chapter	1
Pages	3-23
Number of pages	21
ISBN (Electronic)	978-1-0716-3766-1
ISBN (Print)	978-1-0716-3768-5
DOIs	https://doi.org/10.1007/978-1-0716-3766-1_1
Publication status	Published - 20 Mar 2024

Publication series

Series	Methods in molecular biology (Clifton, N.J.)
Volume	2784
ISSN	1064-3745

Bibliographical note

Publisher Copyright:
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

All Science Journal Classification (ASJC) codes

Molecular Biology
Genetics

Access to Document

10.1007/978-1-0716-3766-1_1

Cite this

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Single-Molecule Fluorescent In Situ Hybridization (smFISH) for RNA Detection in Bacteria. / Ciolli Mattioli, Camilla ; Avraham, Roi.
Fluorescence In Situ Hybridization (FISH): Methods and Protocols. ed. / Gal Haimovich. Humana Press, 2024. p. 3-23 (Methods in molecular biology (Clifton, N.J.), Vol. 2784).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

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Single-Molecule Fluorescent In Situ Hybridization (smFISH) for RNA Detection in Bacteria

Abstract

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Bibliographical note

All Science Journal Classification (ASJC) codes

Access to Document

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