SSNMR of biosilica-entrapped enzymes permits an easy assessment of preservation of native conformation in atomic detail

Marco Fragai; Claudio Luchinat; Tommaso Martelli; Enrico Ravera; Irit Sagi; Inna Solomonov; Yael Udi

doi:10.1039/c3cc46896h

SSNMR of biosilica-entrapped enzymes permits an easy assessment of preservation of native conformation in atomic detail

Marco Fragai, Claudio Luchinat^*, Tommaso Martelli, Enrico Ravera, Irit Sagi, Inna Solomonov, Yael Udi

^*Corresponding author for this work

Department of Immunology and Regenerative Biology

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

Bioinspired silica coprecipitates of enzymes find a wide range of applications for analysis and catalysis in industrial and academic research. Here we used SSNMR as a proxy for the 3D structure of enzymes trapped in bioinspired silica. We show that it is easy to assess whether the enzymes retain their native conformation in atomic detail. We thus propose SSNMR as a rapid and reliable tool to analyze this kind of samples at high resolution.

Original language	English
Pages (from-to)	421-423
Number of pages	3
Journal	Chemical Communications
Volume	50
Issue number	4
DOIs	https://doi.org/10.1039/c3cc46896h
Publication status	Published - 5 Dec 2014

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
General Chemistry
Ceramics and Composites
Metals and Alloys
Materials Chemistry
Surfaces, Coatings and Films
Catalysis

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abstract = "Bioinspired silica coprecipitates of enzymes find a wide range of applications for analysis and catalysis in industrial and academic research. Here we used SSNMR as a proxy for the 3D structure of enzymes trapped in bioinspired silica. We show that it is easy to assess whether the enzymes retain their native conformation in atomic detail. We thus propose SSNMR as a rapid and reliable tool to analyze this kind of samples at high resolution.",

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AU - Luchinat, Claudio

AU - Martelli, Tommaso

AU - Ravera, Enrico

AU - Sagi, Irit

AU - Solomonov, Inna

AU - Udi, Yael

PY - 2014/12/5

Y1 - 2014/12/5

N2 - Bioinspired silica coprecipitates of enzymes find a wide range of applications for analysis and catalysis in industrial and academic research. Here we used SSNMR as a proxy for the 3D structure of enzymes trapped in bioinspired silica. We show that it is easy to assess whether the enzymes retain their native conformation in atomic detail. We thus propose SSNMR as a rapid and reliable tool to analyze this kind of samples at high resolution.

AB - Bioinspired silica coprecipitates of enzymes find a wide range of applications for analysis and catalysis in industrial and academic research. Here we used SSNMR as a proxy for the 3D structure of enzymes trapped in bioinspired silica. We show that it is easy to assess whether the enzymes retain their native conformation in atomic detail. We thus propose SSNMR as a rapid and reliable tool to analyze this kind of samples at high resolution.

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DO - 10.1039/c3cc46896h

M3 - Article

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JO - Chemical Communications

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IS - 4

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SSNMR of biosilica-entrapped enzymes permits an easy assessment of preservation of native conformation in atomic detail

Abstract

All Science Journal Classification (ASJC) codes

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