Super-resolved coherent anti-Stokes Raman scattering microscopy by coherent image scanning

Anna Zhitnitsky, Elad Benjamin, Ora Bitton, Dan Oron*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

We present super-resolved coherent anti-Stokes Raman scattering (CARS) microscopy by implementing phase-resolved image scanning microscopy, achieving up to two-fold resolution increase as compared with a conventional CARS microscope. Phase-sensitivity is required for the standard pixel-reassignment procedure since the scattered field is coherent, thus the point-spread function is well-defined only for the field amplitude. We resolve the complex field by a simple add-on to the CARS setup enabling inline interferometry. Phase-sensitivity offers additional contrast which informs the spatial distribution of both resonant and nonresonant scatterers. As compared with alternative super-resolution schemes in coherent nonlinear microscopy, the proposed method is simple, requires only low-intensity excitation, and is compatible with any conventional forward-detected CARS imaging setup.

Original languageEnglish
Article number10073
JournalNature Communications
Volume15
DOIs
Publication statusPublished - 21 Nov 2024

Funding

We thank Shira Cohen and Professor Philipp Selenko for preparing and providing HEK cell samples. We thank Irit Goldian for her assistance in obtaining AFM images. D.O. is the incumbent of the Harry Weinrebe professorial chair of laser physics.

All Science Journal Classification (ASJC) codes

  • General Chemistry
  • General Biochemistry,Genetics and Molecular Biology
  • General Physics and Astronomy

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