Testing human sperm chemotaxis: How to detect biased motion in population assays

Leah Armon; S. Roy Caplan; Michael Eisenbach; Benjamin M. Friedrich

doi:10.1371/journal.pone.0032909

Testing human sperm chemotaxis: How to detect biased motion in population assays

Leah Armon, S. Roy Caplan, Michael Eisenbach, Benjamin M. Friedrich

Department of Biomolecular Sciences

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14 Citations (Scopus)

Abstract

Biased motion of motile cells in a concentration gradient of a chemoattractant is frequently studied on the population level. This approach has been particularly employed in human sperm chemotactic assays, where the fraction of responsive cells is low and detection of biased motion depends on subtle differences. In these assays, statistical measures such as population odds ratios of swimming directions can be employed to infer chemotactic performance. Here, we report on an improved method to assess statistical significance of experimentally determined odds ratios and discuss the strong impact of data correlations that arise from the directional persistence of sperm swimming.

Original language	English
Article number	e32909
Journal	PLoS ONE
Volume	7
Issue number	3
DOIs	https://doi.org/10.1371/journal.pone.0032909
Publication status	Published - 8 Mar 2012

All Science Journal Classification (ASJC) codes

General
General Biochemistry,Genetics and Molecular Biology
General Agricultural and Biological Sciences

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Testing human sperm chemotaxis: How to detect biased motion in population assays

Abstract

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