The role of electrons’ spin in DNA oxidative damage recognition

Qirong Zhu; Yael Kapon; Aaron M. Fleming; Suryakant Mishra; Kakali Santra; Francesco Tassinari; Sidney R. Cohen; Tapan Kumar Das; Yutao Sang; Deb K. Bhowmick; Cynthia J. Burrows; Yossi Paltiel; Ron Naaman

doi:10.1016/j.xcrp.2022.101157

The role of electrons’ spin in DNA oxidative damage recognition

Qirong Zhu, Yael Kapon, Aaron M. Fleming, Suryakant Mishra, Kakali Santra, Francesco Tassinari, Sidney R. Cohen, Tapan Kumar Das, Yutao Sang, Deb K. Bhowmick, Cynthia J. Burrows^*, Yossi Paltiel^*, Ron Naaman^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Formation of 8-oxo-7,8-dihydro-2′-deoxyguanosine (OG) is one of the most common forms of DNA oxidative damage found in human cells. Although this damage is prevalent in many disease states, it only marginally influences the structure and stability of double-stranded DNA (dsDNA). Therefore, it is a challenge to establish the mechanism by which this damage is detected by repair enzymes. We investigated the position-dependent effect of the damage on the interactions between dsDNA and oligopeptides using atomic force microscopy. The results were confirmed by monitoring the spin and location-dependent polarizability of the damaged DNA, applying a Hall device. The observations suggest that the interaction of peptide with DNA depends on oxidative damage in the DNA and on its location relative to the point of contact between the peptide and the DNA. Hence, a remote search mechanism for damage in DNA is possible.

Original language	English
Article number	101157
Journal	Cell Reports Physical Science
Volume	3
Issue number	12
Early online date	28 Nov 2022
DOIs	https://doi.org/10.1016/j.xcrp.2022.101157
Publication status	Published - 21 Dec 2022

Funding

The authors acknowledge the support from Dr. Tatyana Bendikov and Dr. Yifeng Cao. R.N. acknowledges the support of the Israel Science Foundation and of the Air Force Office of Scientific Research Grant FA9550-21-1-0418 . C.J.B. thanks the U.S. National Science Foundation ( CHE-1808475 ) for supporting this work.

All Science Journal Classification (ASJC) codes

General Chemistry
General Materials Science
General Engineering
General Energy
General Physics and Astronomy

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10.1016/j.xcrp.2022.101157Licence: CC BY-NC-ND

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title = "The role of electrons{\textquoteright} spin in DNA oxidative damage recognition",

abstract = "Formation of 8-oxo-7,8-dihydro-2′-deoxyguanosine (OG) is one of the most common forms of DNA oxidative damage found in human cells. Although this damage is prevalent in many disease states, it only marginally influences the structure and stability of double-stranded DNA (dsDNA). Therefore, it is a challenge to establish the mechanism by which this damage is detected by repair enzymes. We investigated the position-dependent effect of the damage on the interactions between dsDNA and oligopeptides using atomic force microscopy. The results were confirmed by monitoring the spin and location-dependent polarizability of the damaged DNA, applying a Hall device. The observations suggest that the interaction of peptide with DNA depends on oxidative damage in the DNA and on its location relative to the point of contact between the peptide and the DNA. Hence, a remote search mechanism for damage in DNA is possible.",

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AU - Kapon, Yael

AU - Fleming, Aaron M.

AU - Mishra, Suryakant

AU - Santra, Kakali

AU - Tassinari, Francesco

AU - Cohen, Sidney R.

AU - Das, Tapan Kumar

AU - Sang, Yutao

AU - Bhowmick, Deb K.

AU - Burrows, Cynthia J.

AU - Paltiel, Yossi

AU - Naaman, Ron

PY - 2022/12/21

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AB - Formation of 8-oxo-7,8-dihydro-2′-deoxyguanosine (OG) is one of the most common forms of DNA oxidative damage found in human cells. Although this damage is prevalent in many disease states, it only marginally influences the structure and stability of double-stranded DNA (dsDNA). Therefore, it is a challenge to establish the mechanism by which this damage is detected by repair enzymes. We investigated the position-dependent effect of the damage on the interactions between dsDNA and oligopeptides using atomic force microscopy. The results were confirmed by monitoring the spin and location-dependent polarizability of the damaged DNA, applying a Hall device. The observations suggest that the interaction of peptide with DNA depends on oxidative damage in the DNA and on its location relative to the point of contact between the peptide and the DNA. Hence, a remote search mechanism for damage in DNA is possible.

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The role of electrons’ spin in DNA oxidative damage recognition

Abstract

Funding

All Science Journal Classification (ASJC) codes

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